Preparing for NHP Processing🔗

10.5T NHP ABCD BIDS Pipeline Synth

For this pipeline, the data first needs to be converted and properly orientated before being ran.

1. Get DICOMs from s3 bucket (s3://zlab-nhp)

2. Convert DICOMs to BIDS and apply NORDIC

   • Use the Dcm2bids3 NORDIC wrapper -- needs a pair of 10.5T-specific config files to run, and use --keep-non-nordic when calling nordicsbatch.sh in the post-op command
   • Confirm the number of noise volumes per run-- for the Z-Lab 10.5T data, this is usually 5

3. Correct the orientation of the images. NOTE: These steps should be correct for data aquired mid-2021 onward, older data may be orientated differently. Please check with Thomas (tmadison@umn.edu) if you encounter data that does not match the descriptions below after performing the orientation correction.

   To correct the fmap and func files: - Naviagate into the proper directory: cd /projects/standard/faird/shared/code/internal/utilities/zlab_10p5T_nhp_processing/orientation_fix

   • Load MATLAB: module load matlab

   • Run these commands to fix the orientations

   for n in <...>/fmap/*nii.gz; do matlab -nodisplay -nosplash -r "sphinx2headfirstsupineforfmapandfunc('${n}'); exit"; fslreorient2std ${n}; fslswapdim ${n} x y -z ${n}; done

   for n in <...>/func/*nii.gz; do matlab -nodisplay -nosplash -r "sphinx2headfirstsupineforfmapandfunc('${n}'); exit"; fslreorient2std ${n}; fslswapdim ${n} x y -z ${n}; done

   • Load FSL: module load fsl

   • Open an image to check the orientation: fslview_deprecated <fmap/func-image>

   • From left to right, the panels should show the sagittal, coronal, and axial view

   • The axis labels (S/I, A/P, L/R) are correct. L and R labels should be on the right and left sides of the panel, respectively (i.e. they are on the "wrong" sides) due to the Right-to-Left xorient

   • The brain in the center panel should appear "right side up"

   • Check the sform information, should be similar to below output: fslhd <fmap/fun-image>

         sform_name Scanner Anat
         sform_code 1
         sto_xyz:1 -0.753247 0.000000 0.000000 39.195728 
         sto_xyz:2 0.000000 0.753247 0.000000 -59.425900 
         sto_xyz:3 0.000000 0.000000 0.750000 -3.215199 
         sto_xyz:4 0.000000 0.000000 0.000000 1.000000 
         sform_xorient Right-to-Left
         sform_yorient Posterior-to-Anterior
         sform_zorient Inferior-to-Superior
     

   For anat images, there shouldn't be any correction needed - The "L" and "R" labels should be on the left and right sides respectively (i.e. on their "correct" sides)

   • Check the sform information, which should be similar to below: fslhd <anat-image>

         sform_name Scanner Anat
         sform_code 1
         sto_xyz:1 0.500000 0.000000 0.000000 -63.750000 
         sto_xyz:2 0.000000 0.468750 0.000000 -77.436798 
         sto_xyz:3 0.000000 0.000000 0.468750 -67.006302 
         sto_xyz:4 0.000000 0.000000 0.000000 1.000000 
         sform_xorient Left-to-Right
         sform_yorient Posterior-to-Anterior
         sform_zorient Inferior-to-Superior
     

4. Make T1w brain mask (or use pre-made mask from previous session)

   • Pre-made masks are located at s3://zlab-nhp/macaque_masks

   • Use the dilM_edit.nii.gz versions which have been manually edited to work better with the pipeline

5. Add IntendedFor fields to fmap jsons

6. Now you are ready to run the pipeline (see pipelines for the command)

For questions, suggestions, or to note any errors, post an issue on our Github.